RapamycinFungusV1, Main, Exploration, bibRecord, 000454

The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.

Identifieur interne : 000454 ( Main/Exploration ); précédent : 000453; suivant : 000455

The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.

Auteurs : Jo-Chi Kao [Taïwan] ; Wei-Chun Huangfu [Taïwan] ; Tsung-Ting Tsai [Taïwan] ; Min-Ru Ho [Taïwan] ; Ming-Kai Jhan [Taïwan] ; Ting-Jing Shen [Taïwan] ; Po-Chun Tseng [Taïwan] ; Yung-Ting Wang [Taïwan] ; Chiou-Feng Lin [Taïwan]

Source :

PLoS neglected tropical diseases [ 1935-2735 ] ; 2018.

RBID : pubmed:30125275

Descripteurs français

KwdFr :
- Animaux (MeSH), Animaux allaités (MeSH), Antiparasitaires (MeSH), Antiviraux (MeSH), Concentration en ions d'hydrogène (MeSH), Cricetinae (MeSH), Endocytose (effets des médicaments et des substances chimiques), Endosomes (composition chimique), Endosomes (effets des médicaments et des substances chimiques), Humains (MeSH), Lignée cellulaire (MeSH), Méthode des plages virales (MeSH), Niclosamide (pharmacologie), Souris (MeSH), Souris de lignée ICR (MeSH), Virus de la dengue (physiologie).
MESH :
- composition chimique : Endosomes.
- effets des médicaments et des substances chimiques : Endocytose, Endosomes.
- pharmacologie : Niclosamide.
- physiologie : Virus de la dengue.
- Animaux, Animaux allaités, Antiparasitaires, Antiviraux, Concentration en ions d'hydrogène, Cricetinae, Humains, Lignée cellulaire, Méthode des plages virales, Souris, Souris de lignée ICR.

English descriptors

KwdEn :
- Animals (MeSH), Animals, Suckling (MeSH), Antiparasitic Agents (MeSH), Antiviral Agents (MeSH), Cell Line (MeSH), Cricetinae (MeSH), Dengue Virus (physiology), Endocytosis (drug effects), Endosomes (chemistry), Endosomes (drug effects), Humans (MeSH), Hydrogen-Ion Concentration (MeSH), Mice (MeSH), Mice, Inbred ICR (MeSH), Niclosamide (pharmacology), Viral Plaque Assay (MeSH).
MESH :
- chemical , pharmacology : Niclosamide.
- chemical : Antiparasitic Agents, Antiviral Agents.
- chemistry : Endosomes.
- drug effects : Endocytosis, Endosomes.
- physiology : Dengue Virus.
- Animals, Animals, Suckling, Cell Line, Cricetinae, Humans, Hydrogen-Ion Concentration, Mice, Mice, Inbred ICR, Viral Plaque Assay.

Abstract

BACKGROUND

The antiparasitic agent niclosamide has been demonstrated to inhibit the arthropod-borne Zika virus. Here, we investigated the antiviral capacity of niclosamide against dengue virus (DENV) serotype 2 infection in vitro and in vivo.

PRINCIPLE FINDING

Niclosamide effectively retarded DENV-induced infection in vitro in human adenocarcinoma cells (A549), mouse neuroblastoma cells (Neuro-2a), and baby hamster kidney fibroblasts (BHK-21). Treatment with niclosamide did not retard the endocytosis of DENV while niclosamide was unable to enhance the antiviral type I interferon response. Furthermore, niclosamide did not cause a direct effect on viral replicon-based expression. Niclosamide has been reported to competitively inhibit the mTOR (mammalian target of rapamycin), STAT3 (signal transducer and activator of transcription 3), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathways; however, selective inhibitors of those pathways did not reduce DENV infection. Similar to the vacuolar-type H+-ATPase inhibitor bafilomycin A1, both niclosamide and other protonophores, such as CCCP (carbonyl cyanide m-chlorophenyl hydrazone), and FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), effectively reduced endosomal acidification and viral dsRNA replication. Co-administration of a single dose of niclosamide partially decreased viral replication, viral encephalitis, and mortality in DENV-infected ICR suckling mice.

SIGNIFICANCE

These results demonstrate that niclosamide diminishes viral infection by hindering endosomal acidification.

DOI: 10.1371/journal.pntd.0006715
PubMed: 30125275
PubMed Central: PMC6117097

Affiliations:

Taïwan

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000477
to stream Main, to step Curation: 000477

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.</title>
<author><name sortKey="Kao, Jo Chi" sort="Kao, Jo Chi" uniqKey="Kao J" first="Jo-Chi" last="Kao">Jo-Chi Kao</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Huangfu, Wei Chun" sort="Huangfu, Wei Chun" uniqKey="Huangfu W" first="Wei-Chun" last="Huangfu">Wei-Chun Huangfu</name>
<affiliation wicri:level="1"><nlm:affiliation>Ph.D. Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Ph.D. Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tsai, Tsung Ting" sort="Tsai, Tsung Ting" uniqKey="Tsai T" first="Tsung-Ting" last="Tsai">Tsung-Ting Tsai</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ho, Min Ru" sort="Ho, Min Ru" uniqKey="Ho M" first="Min-Ru" last="Ho">Min-Ru Ho</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jhan, Ming Kai" sort="Jhan, Ming Kai" uniqKey="Jhan M" first="Ming-Kai" last="Jhan">Ming-Kai Jhan</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Shen, Ting Jing" sort="Shen, Ting Jing" uniqKey="Shen T" first="Ting-Jing" last="Shen">Ting-Jing Shen</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tseng, Po Chun" sort="Tseng, Po Chun" uniqKey="Tseng P" first="Po-Chun" last="Tseng">Po-Chun Tseng</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Yung Ting" sort="Wang, Yung Ting" uniqKey="Wang Y" first="Yung-Ting" last="Wang">Yung-Ting Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Chiou Feng" sort="Lin, Chiou Feng" uniqKey="Lin C" first="Chiou-Feng" last="Lin">Chiou-Feng Lin</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:30125275</idno>
<idno type="pmid">30125275</idno>
<idno type="doi">10.1371/journal.pntd.0006715</idno>
<idno type="pmc">PMC6117097</idno>
<idno type="wicri:Area/Main/Corpus">000477</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000477</idno>
<idno type="wicri:Area/Main/Curation">000477</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000477</idno>
<idno type="wicri:Area/Main/Exploration">000477</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.</title>
<author><name sortKey="Kao, Jo Chi" sort="Kao, Jo Chi" uniqKey="Kao J" first="Jo-Chi" last="Kao">Jo-Chi Kao</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Huangfu, Wei Chun" sort="Huangfu, Wei Chun" uniqKey="Huangfu W" first="Wei-Chun" last="Huangfu">Wei-Chun Huangfu</name>
<affiliation wicri:level="1"><nlm:affiliation>Ph.D. Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Ph.D. Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tsai, Tsung Ting" sort="Tsai, Tsung Ting" uniqKey="Tsai T" first="Tsung-Ting" last="Tsai">Tsung-Ting Tsai</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ho, Min Ru" sort="Ho, Min Ru" uniqKey="Ho M" first="Min-Ru" last="Ho">Min-Ru Ho</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jhan, Ming Kai" sort="Jhan, Ming Kai" uniqKey="Jhan M" first="Ming-Kai" last="Jhan">Ming-Kai Jhan</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Shen, Ting Jing" sort="Shen, Ting Jing" uniqKey="Shen T" first="Ting-Jing" last="Shen">Ting-Jing Shen</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tseng, Po Chun" sort="Tseng, Po Chun" uniqKey="Tseng P" first="Po-Chun" last="Tseng">Po-Chun Tseng</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Yung Ting" sort="Wang, Yung Ting" uniqKey="Wang Y" first="Yung-Ting" last="Wang">Yung-Ting Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Chiou Feng" sort="Lin, Chiou Feng" uniqKey="Lin C" first="Chiou-Feng" last="Lin">Chiou-Feng Lin</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei</wicri:regionArea>
<wicri:noRegion>Taipei</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS neglected tropical diseases</title>
<idno type="eISSN">1935-2735</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Animals, Suckling (MeSH)</term>
<term>Antiparasitic Agents (MeSH)</term>
<term>Antiviral Agents (MeSH)</term>
<term>Cell Line (MeSH)</term>
<term>Cricetinae (MeSH)</term>
<term>Dengue Virus (physiology)</term>
<term>Endocytosis (drug effects)</term>
<term>Endosomes (chemistry)</term>
<term>Endosomes (drug effects)</term>
<term>Humans (MeSH)</term>
<term>Hydrogen-Ion Concentration (MeSH)</term>
<term>Mice (MeSH)</term>
<term>Mice, Inbred ICR (MeSH)</term>
<term>Niclosamide (pharmacology)</term>
<term>Viral Plaque Assay (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Animaux allaités (MeSH)</term>
<term>Antiparasitaires (MeSH)</term>
<term>Antiviraux (MeSH)</term>
<term>Concentration en ions d'hydrogène (MeSH)</term>
<term>Cricetinae (MeSH)</term>
<term>Endocytose (effets des médicaments et des substances chimiques)</term>
<term>Endosomes (composition chimique)</term>
<term>Endosomes (effets des médicaments et des substances chimiques)</term>
<term>Humains (MeSH)</term>
<term>Lignée cellulaire (MeSH)</term>
<term>Méthode des plages virales (MeSH)</term>
<term>Niclosamide (pharmacologie)</term>
<term>Souris (MeSH)</term>
<term>Souris de lignée ICR (MeSH)</term>
<term>Virus de la dengue (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Niclosamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antiparasitic Agents</term>
<term>Antiviral Agents</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Endosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Endosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Endocytosis</term>
<term>Endosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Endocytose</term>
<term>Endosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Niclosamide</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la dengue</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Dengue Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Animals, Suckling</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>Humans</term>
<term>Hydrogen-Ion Concentration</term>
<term>Mice</term>
<term>Mice, Inbred ICR</term>
<term>Viral Plaque Assay</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Animaux allaités</term>
<term>Antiparasitaires</term>
<term>Antiviraux</term>
<term>Concentration en ions d'hydrogène</term>
<term>Cricetinae</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Méthode des plages virales</term>
<term>Souris</term>
<term>Souris de lignée ICR</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>The antiparasitic agent niclosamide has been demonstrated to inhibit the arthropod-borne Zika virus. Here, we investigated the antiviral capacity of niclosamide against dengue virus (DENV) serotype 2 infection in vitro and in vivo.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>PRINCIPLE FINDING</b>
</p>
<p>Niclosamide effectively retarded DENV-induced infection in vitro in human adenocarcinoma cells (A549), mouse neuroblastoma cells (Neuro-2a), and baby hamster kidney fibroblasts (BHK-21). Treatment with niclosamide did not retard the endocytosis of DENV while niclosamide was unable to enhance the antiviral type I interferon response. Furthermore, niclosamide did not cause a direct effect on viral replicon-based expression. Niclosamide has been reported to competitively inhibit the mTOR (mammalian target of rapamycin), STAT3 (signal transducer and activator of transcription 3), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathways; however, selective inhibitors of those pathways did not reduce DENV infection. Similar to the vacuolar-type H+-ATPase inhibitor bafilomycin A1, both niclosamide and other protonophores, such as CCCP (carbonyl cyanide m-chlorophenyl hydrazone), and FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), effectively reduced endosomal acidification and viral dsRNA replication. Co-administration of a single dose of niclosamide partially decreased viral replication, viral encephalitis, and mortality in DENV-infected ICR suckling mice.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE</b>
</p>
<p>These results demonstrate that niclosamide diminishes viral infection by hindering endosomal acidification.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30125275</PMID>
<DateCompleted><Year>2018</Year>
<Month>12</Month>
<Day>21</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>12</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1935-2735</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>12</Volume>
<Issue>8</Issue>
<PubDate><Year>2018</Year>
<Month>08</Month>
</PubDate>
</JournalIssue>
<Title>PLoS neglected tropical diseases</Title>
<ISOAbbreviation>PLoS Negl Trop Dis</ISOAbbreviation>
</Journal>
<ArticleTitle>The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.</ArticleTitle>
<Pagination><MedlinePgn>e0006715</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pntd.0006715</ELocationID>
<Abstract><AbstractText Label="BACKGROUND">The antiparasitic agent niclosamide has been demonstrated to inhibit the arthropod-borne Zika virus. Here, we investigated the antiviral capacity of niclosamide against dengue virus (DENV) serotype 2 infection in vitro and in vivo.</AbstractText>
<AbstractText Label="PRINCIPLE FINDING">Niclosamide effectively retarded DENV-induced infection in vitro in human adenocarcinoma cells (A549), mouse neuroblastoma cells (Neuro-2a), and baby hamster kidney fibroblasts (BHK-21). Treatment with niclosamide did not retard the endocytosis of DENV while niclosamide was unable to enhance the antiviral type I interferon response. Furthermore, niclosamide did not cause a direct effect on viral replicon-based expression. Niclosamide has been reported to competitively inhibit the mTOR (mammalian target of rapamycin), STAT3 (signal transducer and activator of transcription 3), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathways; however, selective inhibitors of those pathways did not reduce DENV infection. Similar to the vacuolar-type H+-ATPase inhibitor bafilomycin A1, both niclosamide and other protonophores, such as CCCP (carbonyl cyanide m-chlorophenyl hydrazone), and FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), effectively reduced endosomal acidification and viral dsRNA replication. Co-administration of a single dose of niclosamide partially decreased viral replication, viral encephalitis, and mortality in DENV-infected ICR suckling mice.</AbstractText>
<AbstractText Label="SIGNIFICANCE">These results demonstrate that niclosamide diminishes viral infection by hindering endosomal acidification.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kao</LastName>
<ForeName>Jo-Chi</ForeName>
<Initials>JC</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>HuangFu</LastName>
<ForeName>Wei-Chun</ForeName>
<Initials>WC</Initials>
<AffiliationInfo><Affiliation>Ph.D. Program for Cancer Molecular Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tsai</LastName>
<ForeName>Tsung-Ting</ForeName>
<Initials>TT</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ho</LastName>
<ForeName>Min-Ru</ForeName>
<Initials>MR</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jhan</LastName>
<ForeName>Ming-Kai</ForeName>
<Initials>MK</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shen</LastName>
<ForeName>Ting-Jing</ForeName>
<Initials>TJ</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tseng</LastName>
<ForeName>Po-Chun</ForeName>
<Initials>PC</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Yung-Ting</ForeName>
<Initials>YT</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Chiou-Feng</ForeName>
<Initials>CF</Initials>
<Identifier Source="ORCID">0000-0003-4396-6086</Identifier>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>08</Month>
<Day>20</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS Negl Trop Dis</MedlineTA>
<NlmUniqueID>101291488</NlmUniqueID>
<ISSNLinking>1935-2727</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000977">Antiparasitic Agents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>8KK8CQ2K8G</RegistryNumber>
<NameOfSubstance UI="D009534">Niclosamide</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000833" MajorTopicYN="N">Animals, Suckling</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000977" MajorTopicYN="N">Antiparasitic Agents</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003716" MajorTopicYN="N">Dengue Virus</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004705" MajorTopicYN="N">Endocytosis</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011992" MajorTopicYN="N">Endosomes</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008813" MajorTopicYN="N">Mice, Inbred ICR</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009534" MajorTopicYN="N">Niclosamide</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010948" MajorTopicYN="N">Viral Plaque Assay</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<CoiStatement>The authors have declared that no competing interests exist.</CoiStatement>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>02</Month>
<Day>23</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>07</Month>
<Day>23</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2018</Year>
<Month>08</Month>
<Day>30</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2018</Year>
<Month>8</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>12</Month>
<Day>24</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
<Month>8</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">30125275</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pntd.0006715</ArticleId>
<ArticleId IdType="pii">PNTD-D-18-00285</ArticleId>
<ArticleId IdType="pmc">PMC6117097</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 2017 Apr 28;91(10):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28298600</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antimicrob Agents Chemother. 2015 Feb;59(2):1100-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25487800</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2016 Nov;135:81-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27742486</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomed Pharmacother. 2017 Jul;91:1152-1159</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28531943</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2016 Oct;22(10):1101-1107</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27571349</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2016 Jun 09;6:27670</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27279150</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):19055-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20956291</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2017 May 12;91(11):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28298606</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2017 Aug;27(8):1046-1064</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28685770</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2017 Jul 31;7(1):6910</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28761128</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2011 Feb;3(2):160-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22049308</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Virol. 2014 Dec;9:134-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25462445</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Signal. 2018 Jan;41:89-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28389414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biomed Sci. 2013 Sep 08;20:65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24011333</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Apr 25;496(7446):504-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23563266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Negl Trop Dis. 2014 Aug 07;8(8):e3084</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25101828</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2016 May 05;8(5):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27164125</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2012;8(10):e1002976</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23133371</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2010 Nov 18;8(5):422-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21075353</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2013 Dec 16;5(12):3192-212</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24351799</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2015 Apr;1848(4):1041-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25559317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2012 May 18;287(21):17530-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22474287</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2008 Aug 15;378(1):193-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18571214</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2017 Feb;138:68-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27939840</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2008 Aug 22;373(2):319-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18573235</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2011 Jul 09;(53):e3168</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21775958</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2011 Sep;3(9):1739-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21994804</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2017 Jul;143:218-229</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28461069</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antimicrob Agents Chemother. 2004 Jul;48(7):2693-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15215127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antimicrob Agents Chemother. 2014;58(1):110-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24145533</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2002 Jul 26;110(2):163-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12150925</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>F1000Res. 2016 Jan 19;5:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26918159</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2009 Feb;5(2):e1000299</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19214215</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Cells. 2004 Apr;9(4):359-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15066126</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Lett. 2014 Jul 10;349(1):8-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24732808</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Taïwan</li>
</country>
</list>
<tree><country name="Taïwan"><noRegion><name sortKey="Kao, Jo Chi" sort="Kao, Jo Chi" uniqKey="Kao J" first="Jo-Chi" last="Kao">Jo-Chi Kao</name>
</noRegion>
<name sortKey="Ho, Min Ru" sort="Ho, Min Ru" uniqKey="Ho M" first="Min-Ru" last="Ho">Min-Ru Ho</name>
<name sortKey="Ho, Min Ru" sort="Ho, Min Ru" uniqKey="Ho M" first="Min-Ru" last="Ho">Min-Ru Ho</name>
<name sortKey="Huangfu, Wei Chun" sort="Huangfu, Wei Chun" uniqKey="Huangfu W" first="Wei-Chun" last="Huangfu">Wei-Chun Huangfu</name>
<name sortKey="Huangfu, Wei Chun" sort="Huangfu, Wei Chun" uniqKey="Huangfu W" first="Wei-Chun" last="Huangfu">Wei-Chun Huangfu</name>
<name sortKey="Jhan, Ming Kai" sort="Jhan, Ming Kai" uniqKey="Jhan M" first="Ming-Kai" last="Jhan">Ming-Kai Jhan</name>
<name sortKey="Jhan, Ming Kai" sort="Jhan, Ming Kai" uniqKey="Jhan M" first="Ming-Kai" last="Jhan">Ming-Kai Jhan</name>
<name sortKey="Kao, Jo Chi" sort="Kao, Jo Chi" uniqKey="Kao J" first="Jo-Chi" last="Kao">Jo-Chi Kao</name>
<name sortKey="Lin, Chiou Feng" sort="Lin, Chiou Feng" uniqKey="Lin C" first="Chiou-Feng" last="Lin">Chiou-Feng Lin</name>
<name sortKey="Lin, Chiou Feng" sort="Lin, Chiou Feng" uniqKey="Lin C" first="Chiou-Feng" last="Lin">Chiou-Feng Lin</name>
<name sortKey="Shen, Ting Jing" sort="Shen, Ting Jing" uniqKey="Shen T" first="Ting-Jing" last="Shen">Ting-Jing Shen</name>
<name sortKey="Shen, Ting Jing" sort="Shen, Ting Jing" uniqKey="Shen T" first="Ting-Jing" last="Shen">Ting-Jing Shen</name>
<name sortKey="Tsai, Tsung Ting" sort="Tsai, Tsung Ting" uniqKey="Tsai T" first="Tsung-Ting" last="Tsai">Tsung-Ting Tsai</name>
<name sortKey="Tsai, Tsung Ting" sort="Tsai, Tsung Ting" uniqKey="Tsai T" first="Tsung-Ting" last="Tsai">Tsung-Ting Tsai</name>
<name sortKey="Tseng, Po Chun" sort="Tseng, Po Chun" uniqKey="Tseng P" first="Po-Chun" last="Tseng">Po-Chun Tseng</name>
<name sortKey="Tseng, Po Chun" sort="Tseng, Po Chun" uniqKey="Tseng P" first="Po-Chun" last="Tseng">Po-Chun Tseng</name>
<name sortKey="Wang, Yung Ting" sort="Wang, Yung Ting" uniqKey="Wang Y" first="Yung-Ting" last="Wang">Yung-Ting Wang</name>
<name sortKey="Wang, Yung Ting" sort="Wang, Yung Ting" uniqKey="Wang Y" first="Yung-Ting" last="Wang">Yung-Ting Wang</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000454 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000454 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    RapamycinFungusV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:30125275
   |texte=   The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:30125275" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a RapamycinFungusV1

This area was generated with Dilib version V0.6.38.
Data generation: Thu Nov 19 21:55:41 2020. Site generation: Thu Nov 19 22:00:39 2020

	Serveur d'exploration sur la rapamycine et les champignons
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la rapamycine et les champignons

The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.

The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki